junction of cap and stalk) are transferred to Into 2 halves and bits from collar region (i.e. For tissue culture, mushroom after alcohol sterilization is cut longitudinally These slants are then incubated at 25☌ ± 2☌ for 2 weeks to obtain Serially diluted and transferred to sterile potato-dextrose-agar (PDA) or malt-extract-agar Sterilized fresh fruit body on a loop of wire above a petriplate/sterilized paper. Multi-spore culture is made from spore print that can be obtained by hanging a alcohol Pure culture of mushrooms can be prepared either by multi-spore or by tissue culture. Spawn production technology is divided into following steps (Fig. Technique of mother spawn from pure culture mycelium grown on synthetic medium. Wheat, rye and millet grains as substrate for spawn production and are following the standard Today most of the traditional spawn laboratories world over are using The grain spawn was further perfectedīy Stoller in 1962. Mixed easily and provided many inoculum points. Grain spawn had an advantage over manure spawn as it could be The process of making grain spawn was first introduced by the Pennsylvania State Making manure spawn on sterilized horse manure or compost manure. In the begining of 20th century pure mycelial culture were made and used for Run), Mill-track spawn (bricks dried and made from mixture of horse dung, cow dung and Meadows) and Flake spawn (breaking of beds through which mushroom mycelium has Wild rather than made and was referred as Natural or Virgin spawn (from pastures & Selected cultures on suitable agar medium, followed by culturing on sterilized grains andįurther multiplication on grains. ItĮssentially involves preparation of pure culture of mushroom from tissue/spores,Įvaluation of selected cultures for yield, quality and other desirable traits, maintenance of In simple words spawn is grains covered with mushroom mycelium. Selected mushroom cultured on a convenient medium like wheat, pearl millet, sorghum seed required for growing mushroom, is the vegetative mycelium from a
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